Bioelectric Field Thresholds in Vertebrate Tissue Repair: A Cross-System Analysis of Electrodiffusive Transport and Regenerative Propagation

This paper presents a cross-system analysis of bioelectric threshold signaling in vertebrate tissue repair and regeneration, integrating salamander limb regeneration, mammalian wound healing, and zebrafish fin regrowth into a constrained electrodiffusive transport framework. Using independently verified literature and parameter-sourced biophysical reconstruction, the study examines how endogenous and applied electric fields govern regenerative propagation across coupled epithelial and connective tissues.The paper models regenerative signaling using a diffusion-decay transport framework in which the characteristic propagation scale depends on effective ionic diffusion and local electrophysiological relaxation dynamics. Sensitivity analyses demonstrate that the empirically observed ~200 µm depolarization scale reported in zebrafish fin regeneration emerges within biologically plausible parameter space rather than from tuned single-value fitting.Importantly, the framework does not claim that long-duration salamander wound-current persistence directly predicts zebrafish spatial propagation. Instead, the analysis constrains the local decay rates required to support observed regenerative signaling distances and identifies biologically plausible electrophysiological regimes compatible with those observations.The framework is explicitly limited to bioelectric threshold transport and regenerative propagation. No claims are made regarding universality, cognition, quantum consciousness, or non-biological transport systems. All findings are presented as falsifiable, parameter-constrained, and grounded in independently sourced biological literature.