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March 22, 1991Science1,534 citations

Breaking the Diffraction Barrier: Optical Microscopy on a Nanometric Scale

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EBEric BetzigJTJ. K. TrautmanTHT.D. Harris

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Abstract

In near-field scanning optical microscopy, a light source or detector with dimensions less than the wavelength (lambda) is placed in close proximity (lambda/50) to a sample to generate images with resolution better than the diffraction limit. A near-field probe has been developed that yields a resolution of approximately 12 nm ( approximately lambda/43) and signals approximately 10(4)- to 10(6)-fold larger than those reported previously. In addition, image contrast is demonstrated to be highly polarization dependent. With these probes, near-field microscopy appears poised to fulfill its promise by combining the power of optical characterization methods with nanometric spatial resolution.

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Cite This Study

Betzig et al. (1991) studied this question.

synapsesocial.com/papers/6a14bf37a8829aa21862cfd3https://doi.org/10.1126/science.251.5000.1468
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