High-spectral resolution data for determining leaf water content

Key Points

• To evaluate the relationship between high-resolution spectral reflectance and leaf water content while accounting for variations in leaf structure across diverse plant species.
• Measured leaf spectral reflectance across the 400–2500 nm range using a laboratory spectroradiometer on diverse plant species.
• Evaluated relationships between raw reflectance spectra, leaf internal structure, and leaf water content.
• Calculated the first derivative of the reflectance spectrum at the slopes of water absorption band edges.
• Variations in leaf anatomical structure significantly altered standard spectral reflectance measurements.
• The first derivative of reflectance at water absorption band edges correlated strongly with leaf water content.
• First-derivative spectral measurements at absorption edges remained insensitive to structural differences across plant species.