Echocardiography-guided induction of myocardial ischemia/reperfusion in mice resulted in quicker recovery and less inflammation compared to surgical approaches, with comparable infarct size.
Does echocardiography-guided induction of myocardial IR improve recovery time and reduce inflammatory response compared to surgical techniques in mouse models?
Echocardiography-guided induction of myocardial ischemia/reperfusion in mice is a reproducible, high-throughput method that offers animal welfare advantages and reduces surgery-related confounders compared to traditional surgical approaches.
BACKGROUND: Mouse models of myocardial ischemia with subsequent heart failure are common approaches to examine heart failure pathology and possible treatment strategies. We sought to establish a high-throughput approach for echocardiography-guided induction of myocardial ischemia/reperfusion (IR) in mice. METHODS: After visualization of the left coronary artery with high-resolution ultrasound imaging and echocardiographic definition of the level of coronary occlusion, the left anterior descending artery was temporarily occluded with 2 micromanipulator-controlled needles. Functional and molecular changes were assessed and compared with commonly performed surgical techniques. RESULTS: Echocardiography-guided induction of myocardial IR enabled standardized induction of myocardial IR injury with subsequent left ventricular remodeling. Incorporation of various quality control measures throughout the procedure ensured a high success rate and the absence of relevant postinterventional mortality in experienced hands. Compared with surgical approaches, echocardiography-guided induction of myocardial IR showed a quicker recovery time and induced a less pronounced inflammatory response characterized by decreased local and systemic neutrophil counts. Notably, infarct size and subsequent post-myocardial infarction cardiac dysfunction were comparable between methods. The novel procedure was successfully implemented at different academic institutions with imaging expertise and demonstrated high interinstitutional reproducibility. CONCLUSIONS: Echocardiography-guided induction of myocardial IR enables high-throughput induction of myocardial IR injury with precise echocardiographic definition of the occlusion level and immediate evaluation of cardiac function during ischemia. The method provides a more clinically relevant assessment of IR sequelae and offers notable animal welfare advantages by eliminating the need for ventilation and thoracotomy, thereby mitigating potential surgery-related confounders.
Sicklinger et al. (2025) studied Myocardial ischemia/reperfusion injury. Echocardiography-guided induction of myocardial ischemia/reperfusion vs. Commonly performed surgical techniques was evaluated on Infarct size, cardiac dysfunction, recovery time, and inflammatory response. Echocardiography-guided induction of myocardial ischemia/reperfusion in mice resulted in quicker recovery and less inflammation compared to surgical approaches, with comparable infarct size.
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