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November 20, 1992Science523 citations

Map-Based Cloning of a Gene Controlling Omega-3 Fatty Acid Desaturation in Arabidopsis

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VAVincent ArondelBLBertrand LemieuxIHInhwan Hwang

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Abstract

A gene from the flowering plant Arabidopsis thaliana that encodes an omega-3 desaturase was cloned on the basis of the genetic map position of a mutation affecting membrane and storage lipid fatty acid composition. Yeast artificial chromosomes covering the genetic locus were identified and used to probe a seed complementary DNA library. A complementary DNA clone for the desaturase was identified and introduced into roots of both wild-type and mutant plants by Ti plasmid-mediated transformation. Transgenic tissues of both mutant and wild-type plants had significantly increased amounts of the fatty acid produced by this desaturase.

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Cite This Study

Arondel et al. (1992) studied this question.

synapsesocial.com/papers/6a1e7e2a7f6e8bbb2368feb9https://doi.org/10.1126/science.1455229
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