Background and Aim: Brain cancer is a leading cause of mortality, posing significant treatment challenges. CircRNAs are emerging as innovative biomarkers and therapeutic targets for various diseases, including cancers. This study evaluates the half maximal inhibitory concentration (IC50) of Vitamin C, Ag, and TiO2 nanoparticles (NPs) against the GL-261 tumor cell line and their impact on expression of hsacirc₀001518 in cancerous and healthy mice. This investigation aims to determine the impact of Vitamin C antioxidants and Ag and TiO2 NPs, individually and in combination, on the gene expression patterns of CircRNAs in brain cancer. Methods: The GL-261 cell line was cultured and treated with Vitamin C (25, 50, 75, and 100 µg/ml), Ag (11, 24, 42. 24, and 65 µg/ml), and TiO2 (5, 7, 10. 5, and 17. 5 µg/ml) NPs. The MTT test was performed to find cell viability. The expression of investigated circular RNA after treatment with the IC50 of Vitamin C in healthy and brain tumor-bearing mice was assessed by qRT-PCR. Results: The IC50 values after 48h were 81. 25 µg/ml for Vitamin C, 45. 47 µg/ml for Ag NPs, and 8. 73 µg/ml for TiO2 NPs. qRT-PCR results indicated that the combination of antioxidant and NP significantly decreased circ0001518 expression in brain tumor animals more than any single treatment. Conclusion: The combination of TiO2 and Vitamin C, as well as Ag and Vitamin C, significantly alters investigated circ expression and shows potential as a novel therapeutic approach for brain cancer.
Khojastehkiakola et al. (2026) studied this question.