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June 3, 2026Journal of Antimicrobial Chemotherapy0 citations

Influence of TonB-Dependent Receptor on Cefiderocol Resistance in Enterobacter Hormaechei

Influence of a putative TonB-dependent receptor on cefiderocol susceptibility in clinical Enterobacter hormaechei isolates

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Authors

PTPia TurowskiMCMartina CremannsNPNiels Pfennigwerth

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Overview

Randomized trial investigates cefiderocol resistance mechanisms in Enterobacter hormaechei, indicating mutation's role in resistance development.

Key Points

  • To investigate cefiderocol resistance in clinical Enterobacter hormaechei isolates and genetic mechanisms involved, focusing on the TonB-dependent receptor mutations.
  • Exposed clinical E. hormaechei isolates to increasing cefiderocol concentrations using disk diffusion and broth microdilution.
  • Performed whole genome sequencing to identify resistance mutations.
  • Used CRISPR-Cas9 for site-directed mutagenesis to confirm causality.
  • Six spontaneous mutants exhibited FDC MICs from 2 to 16 mg/L.
  • All mutants had mutations in the tonB-dependent receptor gene such as frameshift mutations and premature stop codons.
  • CRISPR-engineered strains with identical mutations showed the same resistance phenotype, verifying the mechanism.

Cite This Study

Turowski et al. (2026) studied this question.

synapsesocial.com/papers/6a1fc550dee9eb8c0dce6bf1https://doi.org/10.1093/jac/dkag193
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