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June 3, 2026Chemical Engineering Journal0 citationsOpen Access

Application of a three-phase fluidized bed electrode for an electro-enzymatic batch process with the peroxygenase AaeUPO

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MAMichael AbtKarlsruhe Institute of TechnologyCKCarolin KienzleKarlsruhe Institute of TechnologyMDMelissa DitzelKarlsruhe Institute of Technology

Key Points

  • This study aims to explore the use of a fluidized bed electrode in enhancing the electro-enzymatic process for hydroxylation reactions.
  • First utilization of a fluidized bed electrode for an electro-enzymatic process.
  • In-situ generation of co-substrates for AaeUPO's reaction.
  • Optimization of H₂O₂ rate and enzyme concentration to improve reaction outcomes.
  • Successfully hydroxylated 4-ethylbenzoic acid to 4-(1-hydroxyethyl)benzoic acid with improved efficiency.
  • Higher current efficiency and yield achieved through enzyme and co-substrate tuning.

Abstract

• First application of a fluidized bed electrode for an electro-enzymatic process. • In-situ co-substrate generation for the H 2 O 2 -dependent peroxygenase Aae UPO. • Hydroxylation of 4-ethylbenzoic acid (EBA) to 4-(1-hydroxyethyl)benzoic acid (HEBA). • Tuning H₂O₂ rate and enzyme amount to boost current efficiency, yield & production.

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Cite This Study

Abt et al. (2026) studied this question.

synapsesocial.com/papers/6a1fc58bdee9eb8c0dce6f34https://doi.org/10.1016/j.cej.2026.177731
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