Saccharomyces cerevisiae β-Glucan Training Induces a Nonclassical PGE 2 − High /NO− Low Macrophage Phenotype in Response to Pseudomonas aeruginosa Exopolysaccharide
Randomized trial reveals altered macrophage behavior in response to exopolysaccharide, suggesting a new therapeutic strategy for chronic inflammation.
Key Points
This research aims to explore how Saccharomyces cerevisiae beta-glucan training affects macrophage responses to Pseudomonas aeruginosa exopolysaccharide.
Peritoneal macrophages from C57BL/6 mice were trained in vitro with beta-glucan.
Macrophages were subsequently stimulated with purified exopolysaccharide from P. aeruginosa isolated from a cystic fibrosis patient.
Cytokine levels, prostaglandin E2, nitric oxide production, and global proteomic profiles were analyzed.
Trained macrophages showed a significant increase in PGE2 production while minimizing NO release
Amplified secretion of TNF-α and IL-6 was observed alongside other pro-inflammatory mediators
Proteomic analysis confirmed upregulation of PGE2 biosynthetic enzymes and downregulation of inducible NO synthase.