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November 1, 1974Journal of Clinical InvestigationOpen Access

Selective Measurement of Two Lipase Activities in Postheparin Plasma from Normal Subjects and Patients with Hyperlipoproteinemia

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Why the study?

Does selective measurement of protamine-inactivated and protamine-resistant lipase activities in postheparin plasma distinguish between hepatic and extra-hepatic lipases in normal subjects and patients with hyperlipoproteinemia?

Population

180 subjects including 100 normal subjects, 12 patients with hyperchylomicronemia, 32 other patients with…

Comparison

Intravenous administration of sodium heparin… vs Normal subjects compared to patients with…

Design

Cross-sectional

Key result

Protamine-inactivated lipase activity was markedly reduced in 12 patients with hyperchylomicronemia (including familial type I) compared to normal subjects, while protamine-resistant lipase was generally normal.

Authors

RKRonald M. KraussRLRobert I. LevyDFDonald S. Fredrickson

Discussion

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Overview

Supports protamine-based postheparin lipase assay to identify extrahepatic deficiency; hypothesis-generating for diagnostic use in hyperchylomicronemia.

Study Design

Type

Cross-Sectional (n=180)

Structured PICO

Does selective measurement of protamine-inactivated and protamine-resistant lipase activities in postheparin plasma distinguish between hepatic and extra-hepatic lipases in normal subjects and patients with hyperlipoproteinemia?

P
Population
180 subjects, including 100 healthy volunteers and 80 patients with various forms of hyperlipoproteinemia or hypothyroidism, evaluated for postheparin plasma lipase activities.
E
Exposure
Intravenous administration of sodium heparin (standard dose 10 U/kg, up to 100 U/kg in special studies) followed by in vitro assay of postheparin plasma with and without protamine sulfate to selectively measure lipase activities.
C
Comparator
Normal subjects compared to patients with various hyperlipoproteinemias and hypothyroidism.
O
Outcome
Protamine-inactivated lipase activity (lipoprotein lipase of extrahepatic origin) and protamine-resistant lipase activity (hepatic lipase) in postheparin plasma.surrogate

Main Result

p-value: p=<0.001

Selective measurement of postheparin lipase activities using protamine sulfate distinguishes between hepatic and extra-hepatic lipases, revealing specific extra-hepatic lipoprotein lipase deficiencies in patients with familial type I hyperlipoproteinemia.

Limitations

  • Small sample sizes in specific patient subgroups
  • Ascertainment was not complete in all families for genetic documentation

Cite This Study

Krauss et al. (1974) conducted a cross-sectional in Hyperlipoproteinemia (n=180). Hyperchylomicronemia (Type I) vs. Normal subjects was evaluated on Protamine-inactivated (extrahepatic) lipase activity (p=<0.001). Protamine-inactivated lipase activity was markedly reduced in 12 patients with hyperchylomicronemia (including familial type I) compared to normal subjects, while protamine-resistant lipase was generally normal.

synapsesocial.com/papers/6a1ff4a435281a23f90dafc9https://doi.org/10.1172/jci107855
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