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Abstract A method is described for simultaneously estimating copper and zinc in serum, urine, and cerebrospinal fluid by atomic absorption spectrophotometry. Standards are prepared in 150 millimolar NaCl solution, and subsequently standards and samples are diluted with n-butanol:water (6:94, by vol). Both ions may be simultaneously determined in as little as 0.5 ml. The procedure is simple, quick, reliable and reproducible.
Meret et al. (1971) studied this question.