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February 18, 2016Journal of Functional Morphology and KinesiologyOpen Access

Oral administration of HE3286 resulted in an almost complete resolution of CVB-induced myocarditis in mice, demonstrating better efficacy than dexamethasone in reducing the inflammatory response.

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Why the study?

Does HE3286 improve histological and immunohistochemical signs of myocarditis in BALB/c mice infected with coxsackie B3 virus compared to dexamethasone or vehicle?

Population

BALB/c mice infected with coxsackie B3 virus (CB3V)

Comparison

HE3286 administered by oral gavage for 18 days vs Dexamethasone administered intraperitoneally or…

Design

Preclinical

Follow-up

18 days

Key result

Oral administration of HE3286 resulted in an almost complete resolution of CVB-induced myocarditis in mice, demonstrating better efficacy than dexamethasone in reducing the inflammatory response.

Authors

PCPaola CastrogiovanniFTFrancesca M. TrovatoMSMarta Anna Szychlinska

Discussion

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Member takes

Overview

Requires clinical validation before any therapeutic consideration; leaves open translation from this murine model.

Structured PICO

Does HE3286 improve histological and immunohistochemical signs of myocarditis in BALB/c mice infected with coxsackie B3 virus compared to dexamethasone or vehicle?

P
Population
BALB/c mice infected with coxsackie B3 virus treated for 18 days to evaluate histological signs of myocarditis.
I
Intervention
HE3286 (17α-ethynyl-5-androstene-3β,7β,17β-triol) administered by oral gavage for 18 days
C
Comparator
Dexamethasone (Dex) administered intraperitoneally or vehicle (HERF405) administered by oral gavage
O
Outcome
Histological signs of CVB-induced myocarditis and immunohistochemical expression of TNF-α, IL-6, MMP9, ADAM10, and HSP-70surrogate

HE3286 showed superior efficacy to dexamethasone in resolving inflammation and tissue remodeling in a murine model of viral myocarditis.

Cite This Study

Castrogiovanni et al. (2016) studied CVB-induced myocarditis. HE3286 vs. Dexamethasone or vehicle (HERF405) was evaluated on Histological signs of CVB-induced myocarditis and immunohistochemical analysis. Oral administration of HE3286 resulted in an almost complete resolution of CVB-induced myocarditis in mice, demonstrating better efficacy than dexamethasone in reducing the inflammatory response.

synapsesocial.com/papers/6a20bf4e2d525c29f3a04605https://doi.org/10.3390/jfmk1010069
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