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The specificity, homogeneity, and availability of large-batch production of liposomes with natural lipids and synthetic lipids have made them an extremely useful tool for the study of diverse cellular phenomena, as well as in medical applications. In many cases, however, the success of the use of liposomes as drug carriers or vaccines and in gene delivery depends entirely on both their formulation and the method of preparation.
Chatterjee et al. (2003) studied this question.