Protein printing with an atomic force sensing nanofountainpen

We demonstrate the direct printing of proteins on a surface using a cantilevered nanopipette as the probe of a scanned probe microscope. Protein features as small as ∼200 nm were directly delivered through the ∼100 nm aperture of the nanopipette by simply contacting the probe with any surface. This allows for the direct connection of this methodology to standard separation techniques so that multiple proteins can be printed through one nanopipette at different locations in ambient conditions.