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Rachitic kidney cytosol is employed in a simple and sensitive method for the determination of 25-hydroxycholecalciferol (25-HCC) in human plasma. The mean plasma 25-HCC level in 40 volunteers was 27.3 ± 11.8 (SD) ng/ml, whereas lifeguards and patients with biliary cirrhosis displayed significantly higher and lower concentrations, respectively.
Haddad et al. (1971) studied this question.