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June 14, 2021eLife34 citationsOpen Access

Myosin-based regulation of twitch and tetanic contractions in mammalian skeletal muscle

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Population

Isolated fast-twitch muscles of mice

Comparison

Single action potential stimulation and… vs Resting muscle

Design

Preclinical

Key result

Electrical stimulation of isolated mouse fast-twitch muscles revealed that activation involves disruption of the helical array of myosin motors, release from the folded conformation, and actin attachment.

Authors

CHCameron HillEBElisabetta BrunelloLFLuca Fusi

Discussion

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Overview

No immediate clinical implications; leaves open translation of myosin activation mechanisms from mouse models.

Structured PICO

P
Population
Isolated extensor digitorum longus muscles from mice studied using time-resolved X-ray diffraction to observe structural changes during contraction.
I
Intervention
Single action potential stimulation ('twitch') and repetitive stimulation
C
Comparator
Resting muscle
O
Outcome
Structural changes in myosin-containing thick filaments measured by time-resolved X-ray diffractionsurrogate

This study reveals the structural basis for myosin-based regulation of twitch and tetanic contractions in mammalian skeletal muscle, including the mechanism of post-tetanic potentiation.

Limitations

  • Low time resolution of the X-ray framing compared to the speed of force development in a single twitch

Cite This Study

Hill et al. (2021) studied this question. Electrical stimulation (twitch and tetanic contractions) vs. Resting state was evaluated on Structural changes in myosin-containing thick filaments measured by X-ray diffraction. Electrical stimulation of isolated mouse fast-twitch muscles revealed that activation involves disruption of the helical array of myosin motors, release from the folded conformation, and actin attachment.

synapsesocial.com/papers/6a22507a00d082f62f972f04https://doi.org/10.7554/elife.68211
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