Key points are not available for this paper at this time.
A rapid technique for the determination of the dissociation constant and the number of binding sites of an enzyme-substrate complex has been developed. The method is based on measuring the rate of dialysis of radioactive substrate from an enzyme-substrate equilibrium mixture. Accurate results can be obtained quickly, thus permitting studies with somewhat labile substances.
Colowick et al. (1969) studied this question.