Randomized trial demonstrates rejuvenation of aged muscle using a microneedle patch with neonatal muscle vesicles, suggesting an effective anti-aging intervention.
Key Points
The research aims to explore the regenerative potential of neonatal muscle-derived extracellular vesicles (NMEVs) in combating age-related skeletal muscle decline.
NMEVs were isolated from neonatal muscle for in vitro and in vivo experiments.
A bilayer microneedle system was developed to deliver NMEVs to aged mice.
Functional analyses measured effects on mitochondrial function, cellular senescence, and lipid accumulation.
NMEVs significantly reduced palmitic acid-induced senescence, mitochondrial dysfunction, and lipid accumulation in C2C12 cells.
In aged mice, the microneedle delivery system enhanced mitochondrial function and reduced muscle aging and fibrosis.
miR-542-3p in NMEVs downregulated Asxl2-PPARγ and activated the AMPK pathway, reducing lipid accumulation and cellular senescence.