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December 18, 2012Proceedings of the National Academy of SciencesOpen Access

Atomic model of the human cardiac muscle myosin filament

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Population

Myosin filaments isolated from human cardiac muscle in the normal (undiseased) relaxed state

Design

Preclinical

Key result

A 28-Å resolution 3D reconstruction of human cardiac muscle myosin filaments revealed axial and azimuthal head perturbations and defined atomic arrangements within the three crown levels.

Authors

HAHind A. AL-KhayatRKRobert W. KenslerJSJohn M. Squire

Discussion

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Overview

Hypothesis-generating for myosin-based cardiomyopathy mechanisms; leaves open clinical translation pending functional validation.

Structured PICO

P
Population
Myosin filaments isolated from human cardiac muscle in the normal (undiseased) relaxed state
I
Intervention
3D single-particle analysis of electron micrograph images
O
Outcome
3D reconstruction and atomic arrangement of myosin headssurrogate

The 3D reconstruction of the human cardiac muscle myosin filament provides structural insights that will aid in understanding the effects of mutations associated with human cardiomyopathies.

Cite This Study

AL-Khayat et al. (2012) studied Normal (undiseased) human cardiac muscle. A 28-Å resolution 3D reconstruction of human cardiac muscle myosin filaments revealed axial and azimuthal head perturbations and defined atomic arrangements within the three crown levels.

synapsesocial.com/papers/6a45abba45fd8cfa32f82915https://doi.org/10.1073/pnas.1212708110
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