Dopamine modified hyaluronic acid and chitosan/gallic acid grafted chitosan based hydrogel with antibacterial and antioxidant property for wound dressing

The healing of chronic wounds remains a complex and challenging clinical issue. Antioxidant and antibacterial treatments play a crucial role in facilitating the wound healing process. Multifunctional hydrogels derived from natural products have shown promising potential in promoting skin tissue regeneration. In this study, a composite multifunctional hydrogel (CS/CSGA-DAHA) was developed by incorporating dopamine-modified hyaluronic acid (DAHA) and gallic acid-modified chitosan (CSGA), crosslinked through a combination of poly-electrolyte interactions and Schiff base reactions. The resulting hydrogel exhibited a porous microstructure, elastic solid-like mechanical behavior, and effective antibacterial activity against both Gram-negative and Gram-positive bacteria. Furthermore, the cytocompatibility of the hydrogel was confirmed through CCK-8 assay and fluorescent imaging analysis after co-cultivation with L929 fibroblast cells. This research introduces an innovative multifunctional hydrogel with promising potential as a wound dressing for various applications in chronic wound management.