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June 1, 2004Journal of Biological ChemistryOpen Access

Functional Characterization of a Trafficking-defective HCN4 Mutation, D553N, Associated with Cardiac Arrhythmia

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Population

Patients suffering from sinus node dysfunction, progressive cardiac conduction disease, and idiopathic…

Design

Preclinical

Key result

The HCN4 D553N mutation caused reduced membranous expression and decreased If currents due to a dominant-negative trafficking defect, linking it to sinus nodal dysfunction and QT prolongation.

Authors

KUKazuo UedaKNKazufumi NakamuraTHTakeharu Hayashi

Discussion

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Overview

Suggests HCN4 sequencing in unexplained sinus node dysfunction with QT prolongation; leaves open clinical translation pending human studies.

Structured PICO

P
Population
Patients with sinus node dysfunction and other arrhythmias were screened for HCN4 mutations, identifying one patient with the D553N mutation for in vitro functional analysis.
E
Exposure
Genetic screening for HCN4 mutations and in vitro functional characterization of the D553N mutation.
O
Outcome
Functional consequences of the D553N mutation on membranous expression and If currents.surrogate

The loss of function of HCN4 due to the D553N mutation is associated with sinus nodal dysfunction and may underlie clinical features of QT prolongation and polymorphic ventricular tachycardia.

Cite This Study

Ueda et al. (2004) studied Sinus node dysfunction, progressive cardiac conduction disease, and idiopathic ventricular fibrillation. HCN4 D553N mutation was evaluated on In vitro functional expression and If currents. The HCN4 D553N mutation caused reduced membranous expression and decreased If currents due to a dominant-negative trafficking defect, linking it to sinus nodal dysfunction and QT prolongation.

synapsesocial.com/papers/6a557d4f0db8f4832a8e0d3fhttps://doi.org/10.1074/jbc.m311953200
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