Nested polymerase chain reaction (nPCR) increased the detection of enteroviruses in clinical samples from patients with aseptic meningitis to 27%, compared to 6% with conventional virus isolation.
Observational (n=366)
Does nested polymerase chain reaction (nPCR) improve the detection of enteroviruses compared to conventional virus isolation in patients with aseptic meningitis?
Nested PCR significantly increases the diagnostic yield for enteroviral infections in aseptic meningitis compared to conventional isolation methods.
Absolute Event Rate: 27% vs 6%
We used a nested polymerase chain reaction (nPCR) to seek evidence for enteroviruses in clinical samples from patients with symptoms of aseptic meningitis. When compared with conventional virus isolation methods on a total of 366 samples collected during 1994-1995, an increase in positivity from 6% to 27% was shown. The results indicate that nPCR would be a valuable aid to the laboratory diagnosis of enteroviral infections as it can detect those enteroviruses that cannot be identified by current isolation methods.
Riding et al. (1996) conducted an observational in Aseptic meningitis (n=366). Nested polymerase chain reaction (nPCR) vs. Conventional virus isolation methods was evaluated on Positivity for enteroviruses. Nested polymerase chain reaction (nPCR) increased the detection of enteroviruses in clinical samples from patients with aseptic meningitis to 27%, compared to 6% with conventional virus isolation.