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March 1, 1966Journal of Lipid Research571 citationsOpen Access

Complete separation of lipid classes on a single thin-layer plate

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CFColette FreemanDWDoreen P. West

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Abstract

A double-development procedure employing first a polar and then a nonpolar solvent system is described for the complete separation by thin-layer chromatography of the main lipid classes encountered in natural lipids. For better quantification, long plates (34 cm) are employed. Diglycerides were separated from cholesterol, 1,2- from 1,3-diglycerides, and monoglycerides from phospholipids.

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Cite This Study

Freeman et al. (1966) studied this question.

synapsesocial.com/papers/6a6bc232c068f641bcf42cadhttps://doi.org/10.1016/s0022-2275(20)39297-x
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