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January 1, 1990Electrophoresis526 citations

Autoradiography using storage phosphor technology

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RJRichard F. JohnstonSPSiobhan C. PickettDBDavid Barker

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Abstract

We describe the application of photostimulable storage phosphor imaging plates to autoradiography of samples labeled with 32P, 14C and 35S. Imaging plates can detect a 32P-labeled sample at an exposure level of 1 disintegration/mm2, and 14C and 35S at 25 disintegrations/mm2. Compared to X-ray film, imaging plates offer increased sensitivity of 15- to 250-fold for 32P and 20- to 100-fold for 14C and 35S. Resolution is in the 0.3 mm range and is therefore suitable for any gel or blot application.

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Cite This Study

Johnston et al. (1990) studied this question.

synapsesocial.com/papers/6a6f397a78a11c550e08e874https://doi.org/10.1002/elps.1150110503
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