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The aim of this study was to investigate the effect of betaine on survival of Lactobacillus plantarum subjected to drying. A defined medium was devised devoid of betaine. Addition of 2 mM betaine to this medium increased the growth rate of cells grown under osmotic stress and also made the organism more tolerant. Cells cultivated under osmotic stress of 0.6 M sodium chloride and in the presence of betaine showed an increased survival after air drying and subsequent vacuum desiccation to a water activity of 0.12.
Kets et al. (1994) studied this question.