Coexpression of the hSloβ subunit significantly alters the biophysical and pharmacological properties of human BK channels, which may explain the diversity of BK channel phenotypes in native tissues.
A human homolog of the large-conductance calcium-activated potassium (BK) channel β subunit ( hSlo β) was cloned, and its effects on a human BK channel ( hSlo ) phenotype are reported. Coexpression of hSlo and hSlo β, in both oocytes and human embryonic kidney 293 cells, resulted in increased Ca 2+ sensitivity, marked slowing of BK channel activation and relaxation, and a significant reduction in slow inactivation. In addition, coexpression changed the pharmacology of the BK channel phenotype: hSlo -mediated currents in oocytes were more sensitive to the peptide toxin iberiotoxin than were hSlo + hSlo β currents, and the potency of blockade by the alkaloid BK blocker tetrandrine was much greater on hSlo + hSlo β-mediated currents compared with hSlo currents alone. No significant differences in the response to charybdotoxin or the BK channel opener NS1619 were observed. Modulation of BK channel activity by phosphorylation was also affected by the presence of the hSlo β subunit. Application of cAMP-dependent protein kinase increased P OPEN of hSlo channels, but decreased P OPEN of most hSlo + hSlo β channels. Taken together, these altered characteristics may explain some of the wide diversity of BK channel phenotypes observed in native tissues.
Dworetzky et al. (1996) studied this question.