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January 1, 1972The Analyst1,428 citations

An improved colour reagent for the determination of blood glucose by the oxidase system

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PTP. Trinder

Key Points

  • To develop a sensitive chromogenic reagent system using 4-aminophenazone and sulphonated 2,4-dichlorophenol for determining hydrogen peroxide generated during glucose oxidation.
  • Coupled 4-aminophenazone with sulphonated 2,4-dichlorophenol to detect hydrogen peroxide produced by glucose oxidase.
  • Quantified chromophore formation spectrophotometrically at 515 nm using 10-mm path length cells in a 4 ml reaction volume.
  • Measurement of 20 µg glucose in a 4 ml volume generated an optical density of 0.61 at 515 nm.
  • Achieved a high molecular absorption coefficient of 22,000 for the resulting colored product.

Abstract

A method involving the use of 4-aminophenazone as a colour coupler with sulphonated 2,4-dichlorophenol is described for determining the hydrogen peroxide produced from glucose with glucose oxidase.The sensitivity of the method is such that 20 µg of glucose in a final volume of 4 ml give an optical density of 0·61 at 515 nm with 10-mm cells, corresponding to a molecular absorption of 22 000.

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Cite This Study

P. Trinder (1972) studied this question.

synapsesocial.com/papers/6a7ca61510d8655be6fab592https://doi.org/10.1039/an9729700142
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