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July 8, 1980Biochemistry79 citations

Phospholamban, activator of the cardiac sarcoplasmic reticulum calcium pump. Physicochemical properties and diagonal purification

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CPChristian J. Le PeuchCentre National de la Recherche ScientifiqueDPDanielle A.‐M. Le PeuchInsermJDJacques DemailleUniversity of Geneva

Key Points

  • To determine the physicochemical properties of phospholamban and establish a diagonal purification method to isolate this cardiac sarcoplasmic reticulum calcium pump regulator.
  • Employed diagonal purification protocols to isolate and purify phospholamban from cardiac sarcoplasmic reticulum preparations.
  • Analyzed the biochemical and physicochemical characteristics of purified phospholamban and its interaction with the calcium transport system.
  • Demonstrated effective isolation and diagonal purification of phospholamban from cardiac sarcoplasmic reticulum membranes.
  • Characterized the physicochemical properties defining phospholamban as an activator of the cardiac calcium pump mechanism.

Abstract

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhospholamban, activator of the cardiac sarcoplasmic reticulum calcium pump. Physicochemical properties and diagonal purificationChristian J. Le Peuch, Danielle A. M. Le Peuch, and Jacques G. DemailleCite this: Biochemistry 1980, 19, 14, 3368–3373Publication Date (Print):July 8, 1980Publication History Published online1 May 2002Published inissue 8 July 1980https://pubs.acs.org/doi/10.1021/bi00555a042https://doi.org/10.1021/bi00555a042research-articleACS PublicationsRequest reuse permissionsArticle Views59Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

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Cite This Study

Peuch et al. (1980) studied this question.

synapsesocial.com/papers/6a7e3740410afd830ac7680ahttps://doi.org/10.1021/bi00555a042
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