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December 1, 2005Journal of Clinical Microbiology100 citationsOpen Access

Development of a Triplex Real-Time PCR Assay for Detection of Panton-Valentine Leukocidin Toxin Genes in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

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RMRyan McDonaldNANick A. AntonishynTHToni Hansen

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Abstract

Community-associated methicillin-resistant Staphylococcus aureus harboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA, nuc, and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA.

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McDonald et al. (2005) studied this question.

synapsesocial.com/papers/6a826f8247f72330e2ffc16fhttps://doi.org/10.1128/jcm.43.12.6147-6149.2005
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