Newborn mouse brain cells expressed maximal levels of coxsackievirus B3 receptors, with approximately 1.8 x 10(4) binding sites per cell, which may relate to their high susceptibility to infection.
The high susceptibility of newborn mouse brain cells to coxsackievirus B3 infection appears related to the preferential expression of viral cellular receptors during late fetal and newborn stages.
This study was designed mainly to determine the relationships between the expression and distribution of the cellular receptor proteins for coxsackievirus B3 (CVB3) and susceptibility of mouse brain cells during fetal development of Balb/c mice. Immunoblot analysis of fetal extracts demonstrated that the CVB3 receptor proteins were first expressed at day 14 of the fetal stage, and that maximal expression of the cellular receptor occurred at near term or newborn stage. Results also suggested that newborn mouse brain tissue expressed much larger quantities of viral receptor proteins, compared to other tissues. In vitro studies showed that both mouse neurons and astrocytes could be infected by two CVB3 strains, pantropic CVB3 Nancy strain (CVB3N) and myocardiotropic CVB3 Woodruff strain (CVB3W). CVB3N, however, replicated and grew to high titer in primary astrocyte cultures and in primary neuron cultures, whereas, primary astrocyte cultures were relatively resistant to CVB3W. Virus binding assays revealed that CVB3N bound faster and in greater amounts to mouse brain cells than CVBW. These two virus strains, however, were found to share the same receptor specificity by virus competition assays. The number of virus binding sites for CVB3 on newborn mouse brain cells was approximately 1.8 x 10(4) per cell. The data suggested that preferential expression of the cellular receptors on newborn mouse brain cells may be related to their high susceptibilities to CVB3 infection.
Xu et al. (1996) studied Coxsackievirus B3 infection. Coxsackievirus B3 was evaluated on Expression and distribution of cellular receptor proteins for CVB3. Newborn mouse brain cells expressed maximal levels of coxsackievirus B3 receptors, with approximately 1.8 x 10(4) binding sites per cell, which may relate to their high susceptibility to infection.