A receptor on YAC-1 mouse T-lymphoma cells bound all six serotypes of group B coxsackieviruses, resembling the HeLa cell receptor protein Rp-a.
The YAC-1 mouse T-lymphoma cell line possesses a receptor for group B coxsackieviruses that resembles the HeLa cell receptor protein Rp-a.
A receptor on YAC-1 cells, a mouse T-lymphoma cell line, bound all six serotypes of the group B coxsackieviruses (CVB). In addition, the cells produced infectious virus. Each of the CVB competed for the same receptor on YAC-1 cells. CVB3 bound relatively slowly to YAC-1 cells (k = 4 x 10(-11) min-1 cell-1), and there were only 500 attachment sites per cell. A rabbit antiserum prepared against the HeLa cell receptor protein Rp-a specifically inhibited the binding of CVB1 and CVB3. A virus-receptor complex with CVB3 could be isolated from detergent (0.5% sodium deoxycholate, 1% Triton X-100)-solubilized YAC-1 plasma membranes. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the iodinated virus-receptor complex revealed a band with the same mobility as Rp-a. The results suggested that the YAC-1 receptor for CVB resembles that of the HeLa cell receptor.
Hsu et al. (1989) studied Group B coxsackieviruses infection. A receptor on YAC-1 mouse T-lymphoma cells bound all six serotypes of group B coxsackieviruses, resembling the HeLa cell receptor protein Rp-a.