Population
Mammalian lymphoid expression system expressing wild-type and mutant lymphocyte voltage-gated K+ channel…
Comparison
Expression of mutant Kv1.3 channels vs Wild-type Kv1.3 channels
Design
Preclinical
Key result
The A413V mutation in the Kv1.3 channel decreased the median inactivation time constant to 4 ms compared to 204 ms for the wild-type channel, supporting a cooperative mechanism of C-type inactivation.
Authors
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Confirms cooperative C-type inactivation in Kv1.3; leaves open translation to native human cardiac channels.
Absolute Event Rate: 4% vs 204%
C-type inactivation of the Kv1.3 channel occurs via a cooperative mechanism where each subunit contributes equally to the activation free energy.
Panyi et al. (1995) studied Voltage-gated K+ channel (Kv1.3) biophysics. A413V mutation in Kv1.3 channel vs. Wild-type Kv1.3 channel was evaluated on Inactivation time constants. The A413V mutation in the Kv1.3 channel decreased the median inactivation time constant to 4 ms compared to 204 ms for the wild-type channel, supporting a cooperative mechanism of C-type inactivation.