Supplemental Figure 2 from Nanobody-Directed CEA-Targeting CAR T Cells Eliminate Gastrointestinal Cancer Xenografts

Supplemental Figure 2. Proliferation and of VHHB30 3rd Generation CAR T Cells in Co-Culture with NB4/NB4CEA Tumor Cells. A. The CAR positivity of VHHB30 third-generation CAR T-cells. B. Total CD3 positive T cells increased 7days after co-culture with VHHHB30 third-generation CAR T-cells while not much in comparison to co-culture with NB4 wild type. C, D. The UTD and CAR T-cells proliferating in coculture with target NB4CEA tumor cells, while the cells decreased in other groups indicating the antigen-dependent stimulation effect of the immune synapse between CAR T-cells and its target. E. NB4CEA tumor cell numbers as a CD33 positive cell population decreased in co-culture with VHHHB30 third-generation CAR T-cells after day 2 indicating the cytotoxicity effect of VHHHB30 third-generation CAR T-cells.