Isolation of Protease Producing Microorganism from Soil and Characterization of Partially Purified Enzyme

Proteases represent a cornerstone class of enzymes in modern biotechnology, constituting approximately 60% of the total enzyme market due to their remarkable versatility and applicability. These specialized biocatalysts function by hydrolyzing peptide bonds in proteins, facilitating their degradation into smaller peptides and amino acids. With an emphasis on process parameter optimization to increase enzyme yield, this thesis investigates the microbial fermentation method of producing proteases. By analyzing how temperature affects protease synthesis, the study determines the ideal circumstances for maximizing enzymatic activity. A number of bioreactor systems are examined for their function in effectively increasing production, including fed-batch fermentation. In commercial enzyme synthesis, the results highlight the significance of regulated environmental conditions and reactor topologies. Thus, this study offers important new information about economical and sustainable methods for producing proteases on a big scale. 21