The root-knot nematode Meloidogyne is an important pest in different Solanaceous plants causing several losses. On the other hand, Hydnora abyssinca is a holoparasitic plant of ethnobotanical value while Tuber indicum and Agaricus impudicus are mushrooms exhibiting promising bioactivities. Hence, potency of hydromethanolic extracts of this plant and the two mushrooms as biocontrol agents was evaluated against Meloidogyne incognita infecting eggplant under screen house conditions. The highest nematicidal activity (86.33% and 97.00%) was recorded after 24 and 48 exposure times, respectively, when T. indicum extract was applied at 2000ppm compared to control. A positive relationship was observed between mortality, concentration, and exposure period. Under screen house conditions, T. indicum extract was also the most reducing treatment in nematode parameter achieved, at 90.15%, 70.27%, and 58.37% in juveniles in soil, galls, and egg masses, respectively at a high concentration. Additionally, reduction in nematode disease improved all plant growth parameters. Analyzing the metabolic profile of T. indicum extract using gas chromatography with a flame ionization detector identified monounsaturated, polyunsaturated and saturated fatty acids. Moreover, GC-MS analysis on metabolites after silylation identified 28 compounds. As far as we know, this is the first study describing nematicidal activity of Hydnora abyssinca and Tuber indicum.
Elkhateeb et al. (Tue,) studied this question.