Optical Coherence Tomography-Based Methodology for In Vivo Visualization and Quantification of Vascular Features and Collagen in Mouse Ovaries

Optical Coherence Tomography Angiography (OCTA) is a non-invasive imaging technique with strong potential for studying ovarian physiology and pathology. Current in vivo methods are often invasive and lack sufficient resolution. Here, we validate OCT for visualizing and quantifying vascular features and collagen structure in mouse ovaries. Our approach provides high-resolution, real-time imaging of ovarian vasculature and collagen networks, with strong accuracy, reproducibility, and sensitivity to age-related changes. Comparison with histology confirms reliability of OCT enables longitudinal assessment and detection of intervention effects. These findings establish OCT as a powerful tool for advancing ovarian aging and reproductive health research.