Asparagus racemosus (Shatavari), a fellow of the Asparagaceae family, grasps a distinct place in Ayurveda medicine owing to its adaptableness in averting and healing a wide range of illnesses. Operative actions prerequisite to be executed to protection of A. racemosus from additional exhaustion and to encourage its supportable growth to encounter both medicinal demands and ecological safeguarding. During present investigation, a protocol was standardized to regenerate plantlets through indirect and direct in vitro organogenesis from nodal segment explants excised from one-year-old plants grown in greenhouse. The treatment of (Bavistin + Streptomycin + Kanamycin) + HgCl2 + Ethanol showed the highest response in respect to percent survival rate (85%). MS medium containing 2.0 mgl-1 2,4-D and 3.0mgl-1NAA induced callus in higher frequencies (74-77 %) from cultured nodal segments. While maximum callus weight (14.88g) was evident on MS medium amended with 2.0 mgl-1TDZ in combination with 1.0 mgl-1NAA. In respect to shoot proliferating efficiency (12.16) with higher shoot length (10.88 cm) was attained on MS medium supplemented with 1.5 mgl-1 BAP in association with 2.0 mgl-1 Kin. Whereas utmost rooting efficiency (84%) with higher root length (6.20±0.08 cm) were evident on ½ strength MS medium fortified with 1.0 mgl-1 IBA. Maximum survival (85%) was recorded in potting mixture comprehending sphagnum peat moss and perlite in 1:1 ratio under greenhouse conditions after five weeks of hardening. The in vitro raised regenerants were acclimatized and established successfully in the field. Although the traits were not scored quantitatively, regenerated plantlets appeared phenotypically normal and true- to- type.
Sharma et al. (Sat,) studied this question.