What type of study is this?

This is a In Vitro Study study.

September 10, 2025

Antiviral In Vitro Screening Using SARS-CoV-2 and MERS-CoV - Cytotoxicity of Drugs v1

Key Points

Antiviral activity is assessed based on the metrics of IC50 and EC50 for drugs.
Assays can utilize two cell lines, A549+ACE2+TMP and VeroE6/TMPRSS2, for testing.
The multiplicity of infection (MOI) is determined by the virus stock titer used.
Cytotoxicity details are essential for medium and high throughput screening in 96- or 384-well plates.

Abstract

The protocol evaluates compounds to determine their antiviral activity against coronaviruses. The antiviral activity is evaluated based on the 50% inhibitory concentration (IC50) and the 50% effective concentration (EC50). Two cell lines can be used for these assays (A549+ACE2+TMP (Invivogen) or VeroE6/TMPRSS2 cells (XenoTech)). The multiplicity of infection (MOI) will depend on the virus stock titer. The incubation time can vary if using a reporter virus. The assay can be performed in 96-well plates (medium throughput screening) or 384-well plates (high throughput screening).

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