The protocol enables accurate quantification of gene expression via qRT-PCR, while outlining protocols for RNA isolation.
Effective cDNA synthesis is emphasized, with detailed procedures integrating RNA isolation methods.
Specific primer sequences are provided, aiding in the accuracy of the qRT-PCR process.
The methodology facilitates reproducibility and standardization in RNA-related experiments.
Abstract
This protocol describes the procedure for RNA isolation, cDNA synthesis and qRT-PCR. Protocol overview A. RNA isolation and cDNA synthesis B. qRT-PCR qRT-PCR primer sequence table