What type of study is this?

This is a Quantitative Study study.

October 1, 2025

Therapeutic drug monitoring of pharmacologically active ampicillin, cefuroxime, meropenem, tazobactam and piperacillin – method validation and results from one-year experience

Key Points

Therapeutic drug monitoring revealed over 50% of patients treated with piperacillin had high beta-lactam levels.
The LC-MS/MS assay achieved an inter-batch imprecision of less than 10% for the analyzed beta-lactams.
Clinical evaluation showed significant numbers of ICU patients exceeding local guidelines for beta-lactam antibiotics.
The method’s precision and annual patient data suggest further research on the clinical relevance of therapeutic drug monitoring.

Abstract

Therapeutic drug monitoring (TDM) is an important tool for securing optimal treatment of drugs like beta-lactam antibiotics in hospitalized patients. Developing accurate and robust methods for quantification of the pharmacologically active fraction is needed. A LC-MS/MS assay, using ultrafiltration for selection of the non-protein bound (i.e. pharmacologically active) fraction followed by a 5.5 min chromatographic separation was developed and validated for simultaneous determination of Ampicillin (AMPI), Cefuroxime (CEFU), Meropenem (MERO) and Piperacillin (PIPE)/Tazobactam (TAZO). Inter-batch imprecision was 50% of patients treated with PIPE (and AMPI), and about 30% treated with MERO and CEFU, exhibited beta-lactam levels higher than the local guidelines for maximum recommended levels. Potential toxic levels were found in several patients. We believe that the analytical method developed in this study represents an accurate measure of the pharmacologically active beta-lactam fraction. Evaluation of patient data after one-year use of the method shows higher than expected beta-lactam levels, highlighting the potential benefit of TDM. More studies are needed to determine the clinical significance of TDM in beta-lactam antibiotics.

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Cite This Study

Christensen et al. (Mon,) studied this question.

synapsesocial.com/papers/68dd89e6fe798ba2fc497f68 https://doi.org/https://doi.org/10.1080/00365513.2025.2565726

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