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EGFRvIII-specific immune responses of Conv. CAR and 7 × 19 CAR-T cells in vitro. A, Surface expression of EGFRvIII was assessed in U87MG glioblastoma cell lines by flow cytometry. Open and filled histograms indicate staining with anti-EGFRvIII Ab and nonstaining, followed by anti–mouse IgG Ab, respectively. B and C, Conv. CAR-T, 7 × 19 CAR-T, or UTD T cells were cocultured with the indicated tumor cells at an effector-to-target (E:T) ratio of 1:1 and 1:5 for 2 days. The number of residual tumor cells (B) and T cells (C) after coculture was analyzed by flow cytometry. Data are shown as mean ± SD of triplicate samples. D, The supernatants of the coculture described in B were harvested, and the concentration of IFNγ was assessed by ELISA. Data are shown as mean ± SD of triplicate samples. **, P P
Ohta et al. (Wed,) studied this question.