The Protective Effect of on The Reproductive System and Some Visceral Organs (Liver, Spleen) Tissues of Female Rats Exposed to High Dose of Zinc Sulphate in Drinking Water

The study examined the effects of high concentrations of zinc sulphate in drinking water on female rats' liver, spleen, and ovaries over eight weeks. The rats were divided into five groups: control, zinc-containing, zinc-sulphate-containing, zinc-sulphate-containing, and zinc-free. As for the objectives of the study, it highlighted the protective and toxic effects of Alchemilla Vulgaris medicinal plant on female rats' liver, spleen, and ovaries tissues from high ZnSO4 concentrations in drinking water. Regarding the method of the study, it involved 30 female albino rats in a pharmacy college/university of Karbala, divided into five groups: control, treatment, treatment, and treatment, with zinc sulphate and Alchemilla vulgaris added to their drinking water. The data was analyzed using one-way ANOVA and SPSS 22.0 software, with four treatment means separated using a "protected" Duncan's analysis at a 0.05 level. The study reached some results, the most important of which are that there are changes in organ tissue, including necrosis in liver cells, congestion in spleen and ovaries, and an increase in progesterone. The study also found that the medicinal plant treated most liver diseases with low side effects, improved ovarian functions, and improved fertility. The study concluded that medicinal plant was used to treat most liver diseases better because its side effects were low. These plants had preventive effects against zinc excess and improved ovarian functions by improving the production of reproductive hormones in females.