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BACKGROUND: The aim of this article is to investigate the effect of luteolin on apoptosis, migration and inflammation of lung cancer cells through regulating Keap1/Nrf2 signaling pathway.METHODS: A549 cells were treated with various concentrations of luteolin, and the MTT assay was used to determine the effective concentrations. Hoechst fluorescence apoptosis staining and wound healing experiments were used to detect the effect of different concentrations of luteolin solution on apoptosis and migration of A549 cells; Western blotting was used to analyze the effect of four concentrations of luteolin on the expression of Nrf2, Keap1, NF-κB, MyD88, TLR4 proteins.RESULTS: With the increase of concentration, luteolin had a significant inhibitory effect on the proliferation and migration of A549 cells, with the best effect observed at 50 μmol/L. Hoechst apoptosis staining found that high concentration of luteolin solution could promote cell apoptosis. Western blot found that high concentration of luteolin could up-regulate the expression of Keap1 protein, and down-regulate the expression of Nrf2, NF-κB, MyD88, TLR4 proteins. There was no significant difference in the effect of 50 μM and 75 μM luteolin solution on the experiment.CONCLUSIONS: With the increase of luteolin solution concentration, the migration effect of non-small cell lung cancer A549 cells is significantly inhibited, and high concentration of luteolin can induce apoptosis of A549 cells, with the best effect at 50 μM luteolin solution. Its possible molecular mechanism is mediated by up-regulating Keap1 and down-regulating Nrf2, significantly reducing the inflammation and cancer characteristics of cancer cells.
QIAN et al. (Sat,) studied this question.