Figure 4 from Molecular and Pathologic Characterization of YAP1-Expressing Small Cell Lung Cancer Cell Lines Leads to Reclassification as SMARCA4-Deficient Malignancies

SCLC-Y cell lines with SMARCA4 loss share a similar transcriptome to SMARCA4-UT. A, Unsupervised hierarchical clustering, based on differentially expressed genes in primary SMARCA4-UT compared with primary SMARCA4-mutant NSCLC identified by Le Loarer and colleagues (24; n = 758 genes), was performed on SCCOHT and lung carcinoma samples cell lines from the CCLE and bulk-RNA sequenced patient samples from Le Loarer and colleagues (24). Inset: SMARCA4-UT cluster from A with cell line and sample annotation. B, mRNA expression of SMARCA4, SMARCA2, and CLDN4 (claudin-4) in cell lines of the SMARCA4-UT cluster, SMARCA4 mutant NSCLC, SMARCA4 proficient NSCLC, and SCLC-A, -N, -P (Supplementary Table S5). Kruskal–Wallis test with a Dunn multiple comparison test; *, P P P P P ≥ 0.05. C, IHC of SMARCA2 and claudin-4 in SMARCA4-WT and SMARCA4-mutant cell line xenografts. Scale bar = 100 μm.