Bioinformatics Analysis of the Actin Interactome

firstₚage Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Bioinformatics Analysis of the Actin Interactome † by Yakov I. MokinYakov I. Mokin SciProfiles Scilit Preprints. org Google Scholar *, Olga I. PovarovaOlga I. Povarova SciProfiles Scilit Preprints. org Google Scholar, Iuliia A. AntifeevaIuliia A. Antifeeva SciProfiles Scilit Preprints. org Google Scholar, Konstantin K. TuroverovKonstantin K. Turoverov SciProfiles Scilit Preprints. org Google Scholar, Irina M. KuznetsovaIrina M. Kuznetsova SciProfiles Scilit Preprints. org Google Scholar and Alexander V. FoninAlexander V. Fonin SciProfiles Scilit Preprints. org Google Scholar Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave. , 194064 St. Petersburg, Russia * Author to whom correspondence should be addressed. † Presented at the 3rd International Electronic Conference on Biomolecules, 23–25 April 2024; Available online: https: //sciforum. net/event/IECBM2024. Proceedings 2024, 103 (1), 58; https: //doi. org/10. 3390/proceedings2024103058 Published: 12 April 2024 (This article belongs to the Proceedings of The 3rd International Electronic Conference on Biomolecules) Download keyboardₐrrowdown Download PDF Download PDF with Cover Download XML Download Epub Versions Notes Keywords: actin; actin-binding proteins; actin interactome; liquid-liquid phase separation; membraneless organelles; Internal disorder; bioinformatics analysis Actin is a key protein of the muscle contraction system. It is present in the cytoplasm, providing motor and framework function through polymerization into F-actin, as well as in the nuclei of all non-muscle cells, where most actin is present in a globular form and participates in processes related to the cell's genetic apparatus, including transcription and DNA repair. Actin interacts with a large number of proteins that form a whole class of actin-binding proteins. Since the functional role of nuclear actin differs significantly from the role of actin in the cell cytoplasm, the goal of this study was to compare the interactome of cytoplasmic and nuclear actin. Using the BioGRID and StringDB databases, proteins that interact with actin experimentally confirmed were selected. Four groups of actin-binding proteins were identified depending on their cellular localization: only cytoplasm, only nucleus, and nucleus and cytoplasm, and others. The analysis of biological processes in the interactome showed that nuclear proteins participate in most key nuclear processes, from DNA damage response to transcription regulation, while cytoplasmic actin-binding proteins are involved in the formation, regulation, and functioning of the cytoskeleton. The analysis of the structure of actin-binding proteins showed a large proportion of internally disordered proteins, most of which are part of membraneless organelles (MLOs). It is known that proteins prone to liquid–liquid phase separation (LLPS) play a key role in the formation of MLOs. Interestingly, although significantly more nuclear proteins are prone to LLPS than cytoplasmic proteins (44% vs. 25%), the drivers of the formation of MLOs in the cytoplasm are significantly (four times) more than in the nucleus. From the pool of actin-binding proteins, 28 clusters were identified, within each of which proteins are capable of forming physical contacts with each other. Author ContributionsY. I. M.: Data curation, Formal analysis, Investigation, Method-ology, Validation, Visualization, Writing—original draft, Writing—review Povarova, O. I. ; Antifeeva, I. A. ; Turoverov, K. K. ; Kuznetsova, I. M. ; Fonin, A. V. Bioinformatics Analysis of the Actin Interactome. Proceedings 2024, 103, 58. https: //doi. org/10. 3390/proceedings2024103058 AMA Style Mokin YI, Povarova OI, Antifeeva IA, Turoverov KK, Kuznetsova IM, Fonin AV. Bioinformatics Analysis of the Actin Interactome. Proceedings. 2024; 103 (1): 58. https: //doi. org/10. 3390/proceedings2024103058 Chicago/Turabian Style Mokin, Yakov I. , Olga I. Povarova, Iuliia A. Antifeeva, Konstantin K. Turoverov, Irina M. Kuznetsova, and Alexander V. Fonin. 2024. "Bioinformatics Analysis of the Actin Interactome" Proceedings 103, no. 1: 58. https: //doi. org/10. 3390/proceedings2024103058 Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.