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In the present study, chitosan nanoparticles were synthesized from Trichoderma asperellum and one commercial product of chitosan (Sigma Aldrich) was taken for comparison. Formation of chitosan nanoparticles (NPs) were confirmed by UV-VIS spectroscopy study with absorption peaks at the range of 310.02 to 342.00 nm. FTIR study showed that synthesized chitosan NP has all the required functional groups like OH, N-H, C-H, C=O, C-O, C-N and P=O. Study on surface properties of NPs by using zetasizer resulted that chitosan NPs synthesized from T. asperellum and commercial product were found to be positively charged and were stable in nature with zeta potential of 9.78 and 17.6 mV respectively. Electron microscopy study showed that the shape of NP as nearly spherical. DLS analysis showed the average size of the chitosan NPs synthesized from T. asperellum and commercial products as 89.03 and 300.10 nm respectively.
Nandini et al. (Mon,) studied this question.