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There is great attention on the development of quick, easy, and sensitive detection techniques for cancer biomarkers. Surface-enhanced Raman spectroscopy (SERS) is a powerful analytical technique that has gained significant attention in the field of cancer research. SERS-based immunoassays are often utilized for the detection of biological structures and molecules in medicine. In the study, a SERS-based immunosensor is developed for the detection of cancer protein biomarkers in serum on a flexible diatomite-based SERS active platform. The flexible SERS active platform is prepared on a regular box tape by coating nanoporous biosilica (diatomite) with AgNPs using the layer-by-layer assembly method. The platform is then modified with antibodies specific to target cancer proteins, such as human epidermal growth factor receptor2 (HER2), mucin4 (MUC 4), and prostate-specific antigen (PSA). The antibody-modified surface is incubated with the cancer proteins spiked in serum at different concentrations, and SERS spectra are obtained after the incubation of Raman probes. The method's sensitivity is evaluated, and the capability to detect protein biomarkers down to 0.1 ng/mL is demonstrated.
Kahraman et al. (Wed,) studied this question.