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Fluorescence imaging is widely used in many different domains, one of which is fluorescence-guided surgery. The temporal behavior called fluorescence lifetime gives deeper insights, making it an additional tool. Current fluorescence-guided surgery imaging typically utilizes two different cameras for natural color and NIR fluorescence imaging, making the system complex and expensive. We propose an alternative approach of sequential RGB+NIR fluorescence lifetime imaging and overlaying the images on each other. Sequential RGB+NIR fluorescence imaging is achieved with a high-speed time-gated camera combined with an RGB LED ring illumination for natural color imaging and a picosecond NIR pulsed laser source for fluorescence lifetime imaging.
Iranian et al. (Wed,) studied this question.