What type of study is this?

This is a Cohort Study study (also classified as: Experimental Study).

October 9, 2025

Structure and assembly of the A-C linker connecting microtubule triplets in centrioles

Key Points

The A-C linker connects microtubule triplets in centrioles, coordinating their assembly.
Using advanced imaging techniques, the study details the structure of the A-C linker and its interactions.
The research integrates multiple methodologies, including biochemistry and cryo–electron microscopy, to uncover centriolar architecture.
These findings highlight the A-C linker's significance in centriolar module organization, shedding light on centriole biology.

Abstract

Centriole assembly involves the coordination of centriolar modules. One module is the A-C linker, an enigmatic protein assembly connecting the A-microtubule of one microtubule triplet to the C-microtubule of the neighboring triplet. Here, we integrated biochemistry, multiscale cryo–electron microscopy, and AlphaFold modeling to investigate the architecture of the centriole. Using an improved centriole isolation method, we determined the structure of the A-C linker bound to microtubule triplets, which revealed how the A-C linker cross-links microtubules and integrates with the B-C junction. We found marked changes in the structure and composition of the A-C linker that correlate with the presence of other centriolar modules, including the pinhead, cartwheel, and inner scaffold. Our findings show that the A-C linker is a highly integrated component of the centriole whose polymorphism may orchestrate the assembly of spatially distinct centriolar modules, and provide a framework for dissecting the biology of centrioles.

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