What question did this study set out to answer?

This research aims to profile bioactive metabolites and assess cytotoxic effects of Alangium salviifolium extracts.

February 2, 2026

Multi-technique comparative analysis of bioactive metabolites in Alangium salviifolium (L.f.) Wangerin leaf, fruit and seed extracts using UV-Vis, FTIR, HPLC, LCMS and cytotoxicity evaluation on A431 cell line

Key Points

This research aims to profile bioactive metabolites and assess cytotoxic effects of Alangium salviifolium extracts.
Qualitative screening for metabolites in leaf, fruit, and seed extracts.
Quantitative assays for flavonoids and phenolics using standard methods.
Cytotoxicity evaluation on A431 cell line with IC50 calculations.
Analysis using UV-Vis, FTIR, HPLC, and LC-MS for compound identification.
Seeds exhibited the highest flavonoids (98.26 mg RE/g) and phenolics (72.42 mg GAE/g).
Fruits were richest in alkaloids (69.46 mg AE/g), while leaves had highest tannins (60.34 mg TAE/g).
Cytotoxicity tests revealed IC50 values of 10 µg/mL for leaf, 8 µg/mL for fruit, and 6.8 µg/mL for seed, nearing doxorubicin's IC50 of 5.4 µg/mL.
LC-MS identified conserved C18 unsaturated fatty acids, phenolics/glycosides, and lipid fragments.

Abstract

This study details the biochemical profiling of leaf, fruit, and seed extracts from Alangium salviifolium (L.f.) Wangerin (Cornaceae). Qualitative screening detected alkaloids, phenolics, flavonoids, tannins, saponins, glycosides, terpenoids, sugars, coumarins, proteins and amino acids. Quantitative assays revealed seeds with highest flavonoids (98.26 mg RE/g) and phenolics (72.42 mg GAE/g), fruits richest in alkaloids (69.46 mg AE/g) and leaves abundant in tannins (60.34 mg TAE/g) and phenolics (65.40 mg GAE/g). UV-Vis, FTIR and HPLC analyses highlighted organ-specific differences. Extracts showed dose-dependent cytotoxicity against A431 cells, with IC50 values of 10, 8 and 6.8 µg/mL for leaf, fruit and seed, respectively-seed extract nearing doxorubicin (5.4 µg/mL). LC-MS identified conserved C18 unsaturated fatty acids (m/z 277-281), phenolics/glycosides (m/z 227-323) and escalating lipid fragments (m/z 455-610) from leaves to fruit/seed. These findings confirm anticancer potential, warranting isolation of active compounds for mechanistic studies and drug development.

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Cite This Study

Karunya et al. (Thu,) studied this question.

synapsesocial.com/papers/6980fd18c1c9540dea80eda5 https://doi.org/https://doi.org/10.1080/14786419.2026.2619417

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